Methods in Cell Biology: The Zebrafish, Biology

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Leslie Wilson, H. William Detrich, Paul Matsudaira, Monte Westerfield, Leonard I. Zon, Associate Professor Harvard Medical School Associate Investigator at Howard Hughes Medical Institute Leonard I Zon
Academic Press, 24.11.1998 - 391 Seiten
This is the first in a two-volume, comprehensive treatment of the methodologies used in researching the Zebrafish, an emerging vertebrate model system. The text includes discussions on development, genetic methodologies, and model applications. Key Features * Details state-of-the-art zebrafish protocols in a single-source reference * Presents methods and reagents in user-friendly format * Delineates critical steps and pitfalls of he procedures * Illustrates techniques with full-color plates * Summarizes many new and interesting developmental mutants * Includes appendices with strain information and a compendium of zebrafish World Wide Web sites * Relevant to clinicians interested in vertebrate models of human congenital diseases
 

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Inhalt

Overview of the Zebrafish System
3
Cell Cycles and Development in the Embryonic Zebrafish
11
The Gastrula Period
24
Clarissa A Henry 179 Department of Zoology University of Washington Seattle
37
Production of Haploid and Diploid Androgenetic Zebrafish Including
45
Analysis of Protein and Gene Expression
63
Strategies to Perturb Zebrafish Development
87
Experiments to Modify the Activity of Specific Gene Products
95
Lilianna SolnicaKrezel 205 Department of Molecular Biology Vanderbilt Univer
205
Kinesinlike Microtubule Motors in Early Development
227
Techniques in Neural Development
253
WholeMount Embryos
269
Growth Control in the Ontogenetic and Regenerating Zebrafish
301
Vascular and Blood Gene Expression
313
Analysis of Hemostasis in the Zebrafish
337
Cell Lineage Tracing in Heart Development
359

Analysis of Zebrafish Development Using Explant Culture Assays
127
Embryonic Axis Formation in the Zebrafish
159
Confocal Microscopic Analysis of Morphogenetic Movements
179
Neurogenesis in Zebrafish Embryos
367
Perspectives
382
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