Molecular improvement of cereal crops
From the pre-historic era to modern times, cereal grains have been the most important source of human nutrition, and have helped sustain the increasing population and the development of human civilization. In order to meet the food needs of the 21st century, food production must be doubled by the year 2025, and nearly tripled by 2050. Such enormous increases in food productivity cannot be brought about by relying entirely on conventional breeding methods, especially on less land per capita, with poor quality and quantity of water, and under rapidly deteriorating environmental conditions.
Complementing and supplementing the breeding of major food crops, such as the cereals, which together account for 66% of the world food supply, with molecular breeding and genetic manipulation may well provide a grace period of about 50 years in which to control population growth and achieve sustainable development. In this volume, leading world experts on cereal biotechnology describe the production and commercialization of the first generation of transgenic cereals designed to substantially reduce or prevent the enormous losses to cereal productivity caused by competition with weeds, and by various pests and pathogens, which is an important first step in that direction.
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Molecular Improvement of Cereal Crops An Introduction
Methods of Genetic Transformation Electroporation and Polyethylene Glycol Treatment
Methods of Genetic Transformation The Gene Gun
Methods of Genetic Transformation Agrobacterium tumefaciens
Expression and Regulation of Transgenes for Selection of Transformants and Modification of Traits in Cereals
Transgenic Cereals Triticum aestivum wheat
Transgenic Cereals Oryza sativa rice
Transgenic Cereals Zea mays maize
Transgenic Cereals Hordeum vulgare L barley
Transgenic Cereals Avena sativa oat
Transgenic Cereals Triticale and Tritordeum
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Acad activity Agrobacterium tumefaciens analysis assays barley bialaphos Bio/Technology Biol biolistic breeding Bregitzer calli callus CaMV 35S cell suspension cereals chromosomes codon crop cultivars Datta dicot DNA delivery efficient electroporation embryogenic embryogenic callus encoding endosperm enzyme explants fertile transgenic frequency Fromm gene expression genetic engineering genetic transformation genome genotypes Golden Promise Gordon-Kamm hybridization immature embryos improvement Indica inflorescences integration intron Japonica Lorz maize cells marker genes Matzke medium methods methylation mg/L microprojectile bombardment molecular monocot monocotyledonous particle bombardment Plant Cell Plant Cell Rep Plant Mol Plant Physiol plant regeneration Plant Sci plasmid Potrykus Proc production of transgenic progeny protein protoplasts recombination reporter gene seed sequences sorghum species stable transformation studies T-DNA target Theor tissue culture tobacco transgenic barley transgenic cereals transgenic maize transgenic plants transgenic rice plants transgenic wheat transient expression Tritordeum uidA Vasil vectors vitro Walbot Wan and Lemaux Zhang