Arabidopsis: A Laboratory ManualCold Spring Harbor Laboratory Press, 2002 - 354 Seiten The thale cress Arabidopsis thaliana is increasingly popular among plant scientists: it is small, easy to grow, and makes flowers, and the sequence of its small and simple genome was recently completed. This is the most complete and authoritative laboratory manual to be published on this model organism and the first to deal with genomic and proteomic approaches to its biology. |
Inhalt
CHAPTER | 3 |
CHAPTER 2 | 19 |
Genetic Analysis of Mutants | 41 |
Urheberrecht | |
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30 minutes acetic acid activity agar Agrobacterium alleles Appendix Arabidopsis thaliana assay buffer CAUTION Centrifuge chemical fume hood cloning concentration containing coverslip diluted distilled H₂O ecotypes EDTA ethanol ethanol series flower fluorescent formamide fume hood fusion gene expression gene of interest genetic genome germination gloves and safety growth harmful by inhalation homozygous hybridization hypocotyl imaging Incubate induced ingestion insertion kanamycin markers master mix Materials medium meristem mg/ml microcentrifuge tube mutant phenotype NaCl overnight Paraplast pellet phenotype pipette Plant Cell plates PMSF primer probe Procedure promoter protein protocol was contributed reaction reagent recombination remove Rinse RNase room temperature root safety glasses seedlings seeds sequence shoot skin absorption slides sodium soil solution staining Step sterile Store sucrose supernatant T-DNA TAIL-PCR tion transcription transfer transformation transgenic trichomes vacuum vectors Wash Wear appropriate gloves western blot wild-type X-Gluc xylene µg/ml