Methods for Affinity-Based Separations of Enzymes and ProteinsMunishwar N. Gupta Springer Science & Business Media, 01.04.2002 - 225 Seiten Shape your heart to front the hour, but dream not that the hour will lost. Alfred Tennyson Locksley Hall Sixty YeO , After Numerous advances in the life sciences have necessitated the need to develop gentle, quick and economical methods for separation of proteins/enzymes. Affmity-based separations, with their high selectivity, have thus come to be used more frequently. In the beginning, we just had atrmity chromatography. Today we have several strategies that employ atrmity interactions for separa tion and analysis. This book is an attempt to provide a short introduction and illustrative protocols for some important and current affinity-based approaches inthe area ofdownstream processingofenzymes/proteins. Today, people from a variety of disciplines have to purify proteins. It is hoped that this book will provide some quick insight into the various available options and guide even a novice (in the area ofprotein separation) to obtain his/her target protein at the level of purity that he/she desires." |
Andere Ausgaben - Alle anzeigen
Methods for Affinity-Based Separations of Enzymes and Proteins Munishwar N. Gupta Eingeschränkte Leseprobe - 2002 |
Methods for Affinity-Based Separations of Enzymes and Proteins Munishwar N. Gupta Eingeschränkte Leseprobe - 2013 |
Häufige Begriffe und Wortgruppen
acid activity adsorbent adsorption affinity adsorbent affinity chromatography affinity interactions affinity ligand agarose alginate beads analyte-receptor antibody applications ATPS B-TR bed expansion bed height binding rate coefficient biosensor breakthrough curve buffer Solution capacity cellulose chitosan chitosan beads column volumes concentration conjugate containing disk dissociation rate coefficient Eluent elution enzyme equilibration ethylene glycol expanded bed expanded bed adsorption extraction flow rate fluidized bed fractal dimension fraction fusion protein glucose HETP IgG₁ immobilized increase inhibitor injection Jurkat cells kinetics lectins Load matrix method mixture ml/min mobile phase molecule monitoring NaCl on-line assay P-TR packed bed particle partitioning PBS pH peptide phosphate buffer polymer precipitation prepared protein purification Protocol pump rate coefficient reaction Reagent recombinant reverse micellar phase sample Selective separation Sepharose sodium Solvent Blend 100 specific SPR biosensor supernatant target protein temperature tion top phase trypsin velocity Wash