Molecular Cellular Microbiology, Band 31Philippe Sansonetti, Arturo Zychlinsky Gulf Professional Publishing, 05.02.2002 - 600 Seiten This volume in the Methods in Microbiology series focuses on the interaction of microorganisms and the host cell, presenting detailed experimental techniques for modern microbiological research. The book focuses on current technical methods, including imaging technologies, cellular biochemistry, and the establishment and exploitation of cell assay systems. Also covered are methods for studying gene expression and detecting virulence genes. By studying the major techniques used to study cross talk between microbes and cells, rather than just presenting systems, this book distinguishes itself as an essential guide for all researchers working in microbiology, cell biology, and immunology. Key Features * Focuses on current technical methods, including imaging technologies, cellular biochemistry, establishment and exploitation of cell assay systems * Covers promising new areas such as global analysis of genome expression and proteomic analysis of cellular components. * Encompasses the most recent and innovative techniques such as microarrays, new experimental models of infection and new cell assay systems * Provides a large array of models covering the various strategies used by pathogens to infect their host * Includes all current methods developed to study gene expression and detect virulence genes. |
Inhalt
Dissecting HostPathogen Molecular Interactions with Microarrays | 19 |
SHE Kaufmann MaxPlanckInstitute for Infection Biology Schumannstr 2122 | 21 |
Lencer Wayne Department of Pediatrics Harvard Medical School Boston MA 02115 | 34 |
Promoter Traps and Related Methods of Identifying Virulence Factors | 37 |
Molecular Methods for Monitoring Bacterial Gene Expression | 55 |
Graham J Boulnois Schroder Ventures Life Sciences Advisers UK Limited | 71 |
Establishment of Primary Cultures | 93 |
Light Microscopy Techniques for Bacterial Cell Biology | 115 |
Intracellular Trafficking and Target Identification | 297 |
Flow Cytometric Analysis of Salmonellacontaining Vacuoles | 319 |
Proteomic Analysis of Phagosomes | 331 |
Studying Trafficking of Intracellular Pathogens | 343 |
Modeling MicrobialEpithelial Interactions in the Intestine | 377 |
Activation of Innate Immune Receptors by Bacterial Products | 397 |
Methods for Studying the Mechanisms of Microbial Entry into | 419 |
Methods for Studying Bacteriainduced Host Cell Death | 439 |
Structure and Function of Pili | 133 |
Measuring and Analysing Invasion of Mammalian Cells | 161 |
Measurement of Bacterial Uptake by Cultured Cells | 179 |
Cell Transfection Permeabilization and Microinjection as Means | 207 |
Modifications of Small GTPbinding Proteins by Bacterial | 225 |
Ian R Booth Professor of Microbiology Department of Molecular Cell Biology | 228 |
Actinbased Motility of Listeria monocytogenes and Shigella flexneri | 245 |
Transport and Intracellular MovementProtein Translocation | 263 |
Alternative Models in Microbial Pathogens | 461 |
Antimicrobial Activity of Host Cells | 477 |
Methods for Studying Infection and Immunity in Drosophila | 507 |
Ultrastructural | 531 |
Combining Gnotobiotic Mouse Models with Functional Genomics | 559 |
588 | |
591 | |
Häufige Begriffe und Wortgruppen
acid actin activity adhesin aerolysin analysis antibodies antigen apoptosis assay bacterial bacterial cells binding buffer caspases cDNA Cell Biol cell lines cellular centrifugation chaperone CNF1 complex concentration containing coverslip culture cytoskeletal detection Differential diluted domain effector endosomes entry epithelial cells Escherichia coli Falkow filter FimH fluorophores fusions gene expression genome gentamicin green fluorescent protein host cells Hultgren hybridization identified Immun incubated induced infection interactions intestinal intracellular invasion isolated kinase labeled lipid Listeria monocytogenes macrophages medium membrane method microarray microbial Microbiol microinjection microscopy Molecular molecules monolayers mouse mutants Natl Acad pathogens periplasm permeabilization phagosomes pili pilus plasmid plate probe Proc protocol purified ratiometric receptor resuspended room temperature Salmonella Salmonella typhimurium samples Shigella signal solution specific staining subunits supernatant target techniques tissue toxin transfection translocation transposon typhimurium uptake virulence factors vitro vivo washed µg ml¹