Molecular Cellular Microbiology
This volume in the Methods in Microbiology series focuses on the interaction of microorganisms and the host cell, presenting detailed experimental techniques for modern microbiological research. The book focuses on current technical methods, including imaging technologies, cellular biochemistry, and the establishment and exploitation of cell assay systems. Also covered are methods for studying gene expression and detecting virulence genes. By studying the major techniques used to study cross talk between microbes and cells, rather than just presenting systems, this book distinguishes itself as an essential guide for all researchers working in microbiology, cell biology, and immunology.
* Focuses on current technical methods, including imaging technologies, cellular biochemistry, establishment and exploitation of cell assay systems
* Covers promising new areas such as global analysis of genome expression and proteomic analysis of cellular components.
* Encompasses the most recent and innovative techniques such as microarrays, new experimental models of infection and new cell assay systems
* Provides a large array of models covering the various strategies used by pathogens to infect their host
* Includes all current methods developed to study gene expression and detect virulence genes.
Was andere dazu sagen - Rezension schreiben
Es wurden keine Rezensionen gefunden.
Dissecting HostPathogen Molecular Interactions with Microarrays
Promoter Traps and Related Methods of Identifying Virulence Factors
Molecular Methods for Monitoring Bacterial Gene Expression
Establishment of Primary Cultures
Light Microscopy Techniques for Bacterial Cell Biology
Structure and Function of Pili
Measuring and Analysing Invasion of Mammalian Cells
Measurement of Bacterial Uptake by Cultured Cells
Proteomic Analysis of Phagosomes
Studying Trafficking of Intracellular Pathogens
Modeling MicrobialEpithelial Interactions in the Intestine
Activation of Innate Immune Receptors by Bacterial Products
Methods for Studying the Mechanisms of Microbial Entry into
Methods for Studying Bacteriainduced Host Cell Death
Alternative Models in Microbial Pathogens
Antimicrobial Activity of Host Cells
Cell Transfection Permeabilization and Microinjection as Means
Modifications of Small GTPbinding Proteins by Bacterial
Actinbased Motility of Listeria monocytogenes and Shigella flexneri
Transport and Intracellular MovementProtein Translocation
Intracellular Trafficking and Target Identification
Flow Cytometric Analysis of Salmonellacontaining Vacuoles
Andere Ausgaben - Alle anzeigen
acid actin activity adhesin analysis antibodies antigen apoptosis Arp2/3 complex assay bacterial binding buffer caspases cDNA Cell Biol cell death cell lines cellular centrifugation chaperone CNF1 complex concentration containing coverslip culture cytokine cytoskeleton cytosol detection diluted domain Drosophila effector endosomes epithelial cells Escherichia coli eukaryotic factor filter fluorescence function fusion gene expression genome gentamicin green fluorescent protein GTPases host cells hybridization identified immune incubated induced infection interactions intestinal intracellular invasion isolated kinase labeled lipid Listeria Listeria monocytogenes macrophages medium membrane method microarray microbial Microbiol Microbiology microscopy Molecular molecules monolayers motility mutants Natl Acad NF-kB pathogens pathway pellet permeabilization phagosomes pilus plate probe Proc protein protocol receptor resuspended room temperature Salmonella samples Shigella signal solution specific staining subunits supernatant surface target tight junctions tissue toxin transcription transfection translocation typhimurium vacuoles virulence vitro vivo washed