Molecular Cellular Microbiology

Cover
Philippe Sansonetti, Arturo Zychlinsky
Gulf Professional Publishing, 19.02.2002 - 600 Seiten
This volume in the Methods in Microbiology series focuses on the interaction of microorganisms and the host cell, presenting detailed experimental techniques for modern microbiological research. The book focuses on current technical methods, including imaging technologies, cellular biochemistry, and the establishment and exploitation of cell assay systems. Also covered are methods for studying gene expression and detecting virulence genes. By studying the major techniques used to study cross talk between microbes and cells, rather than just presenting systems, this book distinguishes itself as an essential guide for all researchers working in microbiology, cell biology, and immunology.

Key Features
* Focuses on current technical methods, including imaging technologies, cellular biochemistry, establishment and exploitation of cell assay systems
* Covers promising new areas such as global analysis of genome expression and proteomic analysis of cellular components.
* Encompasses the most recent and innovative techniques such as microarrays, new experimental models of infection and new cell assay systems
* Provides a large array of models covering the various strategies used by pathogens to infect their host
* Includes all current methods developed to study gene expression and detect virulence genes.
 

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Inhalt

Dissecting HostPathogen Molecular Interactions with Microarrays
19
Promoter Traps and Related Methods of Identifying Virulence Factors
37
Molecular Methods for Monitoring Bacterial Gene Expression
55
Establishment of Primary Cultures
93
Light Microscopy Techniques for Bacterial Cell Biology
115
Structure and Function of Pili
133
Measuring and Analysing Invasion of Mammalian Cells
161
Measurement of Bacterial Uptake by Cultured Cells
179
Proteomic Analysis of Phagosomes
331
Studying Trafficking of Intracellular Pathogens
343
Modeling MicrobialEpithelial Interactions in the Intestine
377
Activation of Innate Immune Receptors by Bacterial Products
397
Methods for Studying the Mechanisms of Microbial Entry into
419
Methods for Studying Bacteriainduced Host Cell Death
439
Alternative Models in Microbial Pathogens
461
Antimicrobial Activity of Host Cells
477

Cell Transfection Permeabilization and Microinjection as Means
207
Modifications of Small GTPbinding Proteins by Bacterial
225
Actinbased Motility of Listeria monocytogenes and Shigella flexneri
245
Transport and Intracellular MovementProtein Translocation
263
Intracellular Trafficking and Target Identification
297
Flow Cytometric Analysis of Salmonellacontaining Vacuoles
319
Methods for Studying Infection and Immunity in Drosophila
507
Ultrastructural
531
Combining Gnotobiotic Mouse Models with Functional Genomics
559
Index
591
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