Techniques in Molecular Systematics and Evolution

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Rob DeSalle, Gonzalo Giribet, Ward Wheeler
Springer Science & Business Media, 01.04.2002 - 410 Seiten
The amount of information that can be obtained by using molecular techniques in evolution, systematics and ecology has increased exponentially over the last ten years. The need for more rapid and efficient methods of data acquisition and analysis is growing accordingly. This manual presents some of the most important techniques for data acquisition developed over the last years. The choice and justification of data analysis techniques is also an important and critical aspect of modern phylogenetic and evolutionary analysis and so a considerable part of this volume addresses this important subject. The book is mainly written for students and researchers from evolutionary biology in search for methods to acquire data, but also from molecular biology who might be looking for information on how data are analyzed in an evolutionary context. To aid the user, information on web-located sites is included wherever possible. Approaches that will push the amount of information which systematics will gather in the
 

Inhalt

The Basic Method
5
Philosophical Perspective
23
Parsimony Analysis
31
Techniques for Analyzing Large Data Sets 70
70
Measures of Support
80
Partitioning of Multiple Data Sets in Phylogenetic Analysis
102
Complex Model Organism Genome Databases
120
A Strategy for Highthroughput
132
Introduction to Part II
175
VI
176
DNA Isolation Procedures
249
Late Pleistocene DNA Extraction and Analysis
288
PCR Methods and Approaches
302
HighThroughput DNA Sequencing for Systematics
328
A Practical Guide for Microsatellite Analysis
351
Determining the Spatial and Temporal Patterns of Developmental
365

Comparative Methods
146
Introduction
158
Analyzing Data at the Population Level
162
Guide to Solutions
395
Index
401
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