Techniques in Molecular Systematics and EvolutionRob DeSalle, Gonzalo Giribet, Ward Wheeler Springer Science & Business Media, 01.04.2002 - 410 Seiten The amount of information that can be obtained by using molecular techniques in evolution, systematics and ecology has increased exponentially over the last ten years. The need for more rapid and efficient methods of data acquisition and analysis is growing accordingly. This manual presents some of the most important techniques for data acquisition developed over the last years. The choice and justification of data analysis techniques is also an important and critical aspect of modern phylogenetic and evolutionary analysis and so a considerable part of this volume addresses this important subject. The book is mainly written for students and researchers from evolutionary biology in search for methods to acquire data, but also from molecular biology who might be looking for information on how data are analyzed in an evolutionary context. To aid the user, information on web-located sites is included wherever possible. Approaches that will push the amount of information which systematics will gather in the |
Inhalt
The Basic Method | 5 |
Philosophical Perspective | 23 |
Parsimony Analysis | 31 |
Techniques for Analyzing Large Data Sets 70 | 70 |
Measures of Support | 80 |
Partitioning of Multiple Data Sets in Phylogenetic Analysis | 102 |
Complex Model Organism Genome Databases | 120 |
A Strategy for Highthroughput | 132 |
Introduction to Part II | 175 |
VI | 176 |
DNA Isolation Procedures | 249 |
Late Pleistocene DNA Extraction and Analysis | 288 |
PCR Methods and Approaches | 302 |
HighThroughput DNA Sequencing for Systematics | 328 |
A Practical Guide for Microsatellite Analysis | 351 |
Determining the Spatial and Temporal Patterns of Developmental | 365 |
Comparative Methods | 146 |
Introduction | 158 |
Analyzing Data at the Population Level | 162 |
Guide to Solutions | 395 |
401 | |
Andere Ausgaben - Alle anzeigen
Techniques in Molecular Systematics and Evolution Rob DeSalle,Gonzalo Giribet,Ward Wheeler Eingeschränkte Leseprobe - 2013 |
Techniques in Molecular Systematics and Evolution Rob DeSalle,Gonzalo Giribet,Ward Wheeler Keine Leseprobe verfügbar - 2014 |
Techniques in Molecular Systematics and Evolution Rob DeSalle,Gonzalo Giribet,Ward Wheeler Keine Leseprobe verfügbar - 2002 |
Häufige Begriffe und Wortgruppen
allele amplification ancestral animals Biol Biology bootstrap branch lengths Bremer support buffer cells Centrifuge characters chemistry clade Cladistics cladogram clones coelacanth collection combined analysis comparative consensus containing culture data sets databases DNA sequences Drosophila EDTA embryos ethanol evolution evolutionary extraction fragments frozen tissue gene genetic genome H₂O homology homoplasy hybridization Incubate isolation labeled Laboratory matrix maximum likelihood method microsatellite mitochondrial molecular systematics monophyly multiple Museum NaCl node Nucleic Acids nucleotide optimal parsimonious parsimonious tree partitions PAUP PCR product PCR reactions pellet phylogenetic phylogenetic analysis phylogeny plant plasmid polymerase population preservation primer probes procedure Proteinase Proteinase K proteins Protocol random regions relationships require Research room temperature sequence data solution species specimens spin steps storage stored studies synapomorphies Syst taxa taxon taxonomic techniques template tion tissue samples traits tube University vertebrate volume Wash weighting µg/ml
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