Confocal Microscopy and Multiphoton Excitation Microscopy: The Genesis of Live Cell ImagingSPIE Press, 2006 - 208 Seiten This text guides you through the principles and practical techniques of confocal and multiphoton microscopy. It also describes the historical connections and parallel inventions that resulted in modern techniques of live cell imaging and their use in biology and medicine. You will find comparisons of different types of confocal and multiphoton microscopes, solutions to the problems one would encounter when using various microscopic techniques, tips on selecting equipment, and an extensive annotated bibliography of additional resources. |
Inhalt
1 | 6 |
1 | 14 |
6 | 28 |
9 | 36 |
Chapter 4 | 42 |
2 | 51 |
Chapter 5 | 55 |
3 | 64 |
The Development of ScanningSlit Confocal Systems | 117 |
The Components of a Confocal Microscope | 135 |
The Development of Nonlinear Spectroscopy | 153 |
Multiphoton Excitation Microscopy | 161 |
Theory and Instrumentation of Multiphoton | 169 |
Remaining Problems Limitations | 181 |
Safety and Cleanliness Considerations | 189 |
205 | |
Andere Ausgaben - Alle anzeigen
Confocal Microscopy and Multiphoton Excitation Microscopy: The Genesis of ... Barry R. Masters Keine Leseprobe verfügbar - 2006 |
Häufige Begriffe und Wortgruppen
Abbe Airy disk aperture axial resolution B. R. Masters back focal plane biology cells and tissues Chapter clinical coherent components condenser confocal micro confocal microscope conjugate contrast microscopy cornea croscope depth discrimination descanning detection detector dichroic mirror disk confocal microscope electronic emission excitation light eyepiece field filter fluorescence microscopy fluorescent molecules fluorescent probes focused illumination light image formation image plane instruments intensity interference invention Köhler illumination laser beam light microscope light source limitations living cells LSCM microscope based microscope objective Minsky multiphoton excitation microscopy Nipkow disk Nipkow disk confocal nonlinear optical observed optical aberrations optical axis optical microscope optical path optical sectioning patent Petràn phase contrast microscope photons pinhole problem quantum real-time reflected light scanning optical scanning-slit confocal microscopes signal slit spatial spectroscopy spherical aberrations Svishchev techniques theory three-dimensional tion two-photon absorption two-photon excitation two-photon excitation microscopy ultraviolet wavelength wide-field
Verweise auf dieses Buch
Handbook of Biomedical Nonlinear Optical Microscopy Barry R. Masters,Peter So Eingeschränkte Leseprobe - 2008 |