Flow Cytometry Protocols
This thoroughly revised and updated edition of a widely used practical guide to flow cytometry describes in step-by-step detail an array of time proven and cutting-edge techniques much needed in today's advanced laboratories. These readily reproducible methods deploy emerging flow cytometry technologies in many new applications, especially in the field of stem cells, functional genomics and proteomics, and microbiology. Here, the aspiring investigator will find methods for the characterization of stem/progenitor cells by monitoring the efflux of fluorescent dyes and the elucidation of signal transduction pathways using phospho-specific antibodies. There are also techniques for monitoring gene transfer and expression using fluorescent protein technology, high throughput screening for discovery of novel protein interactions, phenotypic and functional characterization of T cell subsets and precursors, and microbial flow cytometry, to highlight but some of the many useful procedures.
Was andere dazu sagen - Rezension schreiben
Es wurden keine Rezensionen gefunden.
Andere Ausgaben - Alle anzeigen
Alexa Fluor analysis analyzed annexin antibody antigen apoptosis apoptotic approx assay BD Biosciences beads beam binding bivariate blood bone marrow caspase caspase activation CD34NEG cell cycle cell death cell line cell sorter cell sorting cell suspension cellular centrifuge clones column conjugated culture cytokine cytome cytometer detection dilution diode DsRed ECFP EGFP emission excitation expression EYFP FACS filter flow cytometry fluorochromes gate gene green fluorescent protein He–Ne hematopoietic stem cells histogram Hodull/Rhdull Hoechst human Immunol Incubate intracellular labeled laser light lymphocytes markers membrane methanol Methods microarray mouse Note parameters particles PBMCs pellet peptide permeabilization phenotype PhiPhiLux-G1D2 plate proliferation Protocols R. G. Hawley reagents receptor room temperature sample saponin scFv shown in Fig signal specific staining stock solution Subheading substrate supernatant surface target cells telomere telomere length tissue tube tumor vector wash buffer wavelength yeast