Flow Cytometry ProtocolsTeresa S. Hawley, Robert Hawley Springer Science & Business Media, 03.02.2008 - 434 Seiten Flow cytometry has evolved since the 1940s into a multidisciplinary field incorporating aspects of laser technology, fluid dynamics, electronics, optics, computer science, physics, chemistry, biology, and mathematics. Innovations in instrumentation, development of small lasers, discovery of new fluorochromes/fluorescent proteins, and implementation of novel methodologies have all contributed to the recent rapid expansion of flow cytometry applications. In this thoroughly revised and updated second edition of Flow Cytometry Protocols, time-proven as well as cutting-edge methods are clearly and comprehensively presented by leading experimentalists. In addition to being a valuable reference manual for experienced flow cytometrists, the editors expect this authoritative up-to-date collection to prove useful to investigators in all areas of the biological and biomedical sciences who are new to the subject. The introductory chapter provides an eloquent synopsis of the principles and diverse uses of flow cytometry, beginning with a historical perspective and ending with a view to the future. Chapters 2–22 contain step-by-step protocols of highly practical and state-of-the-art techniques. Detailed instructions and helpful tips on experimental design, as well as selection of reagents and data analysis tools, will allow researchers to readily carry out flow cytometric investigations ranging from traditional phenotypic characterizations to emerging genomics and proteomics applications. Complementing these instructive protocols is a chapter that provides a preview of the next generation of solid-state lasers, and one that describes a rapid means to validate containment of infectious aerosols generated during high-speed sorting (Chapters 23–24). |
Inhalt
2 | |
Shapiro and Gerhard NebevonCaron 3 Multiparameter Data Acquisition and Analysis of Leukocytes | 33 |
Stewart and Sigrid J Stewart 4 Flow Cytometric Analysis of Kinase Signaling Cascades | 45 |
Cytokine Flow Cytometry | 95 |
Use of CellTracking Dyes to Determine Proliferation Precursor | 109 |
Using Flow Cytometry | 125 |
Multiparametric Analysis of Apoptosis by Flow and Image Cytometry | 141 |
Detection and Enrichment of Hematopoietic Stem Cells by Side | 161 |
Pruitt Lawrence M Mielnicki | 239 |
Integrative Flow Cytometric and Microarray Approaches | 259 |
Interactions in Living Cells | 281 |
Sam Lievens José Van der Heyden Els Vertenten Jean Plum | 293 |
Marielle Cavrois Jason Neidleman Martin Bigos | 333 |
Michael G Ormerod 20 Solid Tumor DNA Content Analysis | 345 |
Telomere Length Measurement by Fluorescence In Situ | 385 |
Viable Infectious Cell Sorting in a BSL3 Facility | 419 |
Hematopoietic Stem Cell Characterization by Hoechst 33342 | 181 |
Dooley and Barbara K Oppenlander 12 Multiparameter Flow Cytometry of Fluorescent Protein Reporters | 201 |
Teresa S Hawley Donald J Herbert Shannon S Eaker | 219 |
425 | |
Andere Ausgaben - Alle anzeigen
Häufige Begriffe und Wortgruppen
Alexa Fluor analysis analyzed annexin antibody antigen apoptosis apoptotic approx assay BD Biosciences beads beam binding bivariate blood bone marrow caspase caspase activation CD34NEG cell cycle cell line cell sorting cell suspension cellular centrifuge clones column culture cytokine cytome cytometer detection dilution diode DsRed ECFP EGFP emission excitation expression EYFP FACS filter flow cytometry fluorescent protein fluorochromes forward scatter gate gene green fluorescent green fluorescent protein hematopoietic stem cells histogram Hodull/Rhdull Hoechst human Immunol Incubate intracellular labeled laser lymphocytes markers membrane methanol Methods microarray Molecular mouse Note parameters particles PBMCs pellet peptide permeabilization phenotype Probes Protocols reagents receptor room temperature sample saponin scFv shown in Fig side scatter signal specific staining stock solution Subheading substrate supernatant surface T. S. Hawley target cells telomere telomere length tissue tube tumor vector wash buffer wavelength yeast µg/mL