Arabidopsis Protocols, 2nd EditionJulio Salinas, Jose J. Sanchez-Serrano Springer Science & Business Media, 04.02.2008 - 496 Seiten For several decades, Arabidopsis thaliana has been the organism of choice in the laboratories of many plant geneticists, physiologists, developmental biologists, and biochemists around the world. During this time, a huge amount of knowledge has been acquired on the biology of this plant species, which has resulted in the development of molecular tools that account for much more efficient research. The significance that Arabidopsis would attain in biological research may have been difficult to foresee in the 1980s, when its use in the laboratory started. In the meantime, it has become the model plant organism, much the same way as Drosophila, Caenorhabditis, or mouse have for animal systems. Today, it is difficult to envision research at the cutting edge of plant biology without the use of Arabidopsis. Since the first edition of Arabidopsis Protocols appeared, new developments have fostered an impressive advance in plant biology that prompted us to prepare Arabidopsis Protocols, Second Edition. Completion of the Arabidopsis genome sequence offered for the first time the opportunity to have in hand all of the genetic information required for studying plant function. In addition, the development of whole systems approaches that allow global analysis of gene expression and protein and metabolite dynamics has encouraged scientists to explore new scenarios that are extending the limits of our knowledge. |
Inhalt
Grafting | 39 |
Synchronization Transformation and Cryopreservation | 45 |
Genetic Analysis | 65 |
QTL Analysis | 79 |
EMS Mutagenesis of Arabidopsis | 101 |
Georg Jander 11 HighThroughput TILLING for Arabidopsis | 115 |
Bradley J Till Trenton Colbert Christine Codomo Linda Enns | 127 |
Justin Borevitz 13 Forward Genetic Screening of Insertional Mutants | 137 |
Guido Jach | 275 |
Luciferases as Reporter Genes | 293 |
Christoph Plieth | 307 |
Applications of ChemicalInducible Expression Systems | 329 |
RNA Extraction | 345 |
Willem A Rensink and Samuel P Hazen | 359 |
Preparation and Quality Assesment of RNA From CellSpecific Samples | 367 |
Protein Isolation and SecondDimension Electrophoretic Separation | 381 |
Anja Schneider and Dario Leister | 147 |
PCRBased Screening for Insertional Mutants | 163 |
Cytogenetic Analysis of Arabidopsis | 173 |
Using Information From Public Arabidopsis Databases to Aid Research | 187 |
In Planta AgrobacteriumMediated Transformation by Vacuum Infiltration | 215 |
Min Woo Lee and Yinong Yang | 225 |
Transfection Assays With Protoplasts Containing Integrated Reporter Genes | 237 |
Chloroplast Genetic Engineering Via Organogenesis | 245 |
KyeWon Kim Vincent R Franceschi Laurence B Davin | 263 |
Isolation of Nuclear Proteins | 393 |
Purification and Fractionation of Membranes for Proteomic Analysis | 403 |
Alberto Paradela and Juan Pablo Albar 35 PeptideBased Phosphoproteomics | 421 |
With Immobilized Metal Ion Chromatography | 431 |
Metabolite Profiling in Arabidopsis | 439 |
Hormone Profiling in Arabidopsis | 449 |
459 | |
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Arabidopsis Protocols, 2nd Edition Julio Salinas,Jose J. Sanchez-Serrano Keine Leseprobe verfügbar - 2006 |
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