Molecular EmbryologyPaul T. Sharpe, Ivor Mason Springer Science & Business Media, 02.02.2008 - 756 Seiten Most people have some interest in embryos; this probably results, in part, from their interest in understanding the biological origins of themselves and their offspring and, increasingly, concerns about how environmental change such as pollution might affect human development. Obviously, et- cal considerations preclude experimental studies of human embryos and, c- sequently, the developmental biologist has turned to other species to examine this process. Fortunately, the most significant conclusion to be drawn from the experimental embryology of the last two decades is the manner in which orthologous or closely related molecules are deployed to mediate similar - velopmental processes in both vertebrates and invertebrates. The molecular mechanisms regulating processes fundamental to most animals, such as axial patterning or axon guidance, are frequently conserved during evolution. (It is now widely believed that the differences between phyla and classes are the result of new genes, arising mostly by duplication and divergence of extant sequences, regulating the appearance of derived characters. ) Other vertebrates are obviously most likely to use the same devel- mental mechanisms as humans and, within the vertebrate subphylum, the - parent degree of conservation of developmental mechanism is considerable. It has long been recognized that particular vertebrate species offer either d- tinct advantages in investigating particular stages of development or are - pecially amenable to particular manipulations. No single animal can provide all the answers because not all types of experiments can be carried out on a single species. |
Inhalt
6 | |
23 | |
Cell Grafting and Labeling in Postimplantation Mouse Embryos | 41 |
Production of Transgenic Rodents by the Microinjection | 61 |
Cre Recombinase Mediated Alterations of the Mouse Genome | 101 |
Gene Trapping in Mouse Embryonic Stem Cells | 123 |
Reporter Genes for the Study of Transcriptional Regulation | 159 |
Application of lacZ Transgenic Mice to Cell Lineage Studies | 183 |
Experimental Embryological Methods for Analysis of Neural | 351 |
A Method for Generating Transgenic Frog Embryos | 393 |
Axolotlnewt | 415 |
An Overview of Its Early Development | 431 |
SmallScale MarkerBased Screening for Mutations in Zebrafish | 441 |
Transgenic Zebrafish | 461 |
Microinjection of DNA RNA and Protein into the Fertilized | 487 |
Protochordates | 513 |
Isolation and In Vitro Culture | 201 |
An Overview | 215 |
Incubation and Isolation | 221 |
Grafting Hensens Node | 245 |
Grafting of Somites | 255 |
Notochord Grafts | 265 |
Transplantation of Avian Neural Tissue | 273 |
Grafting of Apical Ridge and Polarizing Region | 280 |
Tissue Recombinations in Collagen Gels | 293 |
QuailChick Chimeras | 305 |
Using Fluorescent Dyes for Fate Mapping Lineage Analysis | 319 |
AMPHIBIAN EMBRYOS | 331 |
Mesoderm Induction Assays | 341 |
Gene Transfer in Avian Embryos Using ReplicationCompetent | 539 |
Subtractive Hybridization and Construction of cDNA Libraries | 555 |
Differential Display of Eukaryotic mRNA | 575 |
RTPCR on Embryos Using Degenerate Oligonucleotide Primers | 591 |
SingleCell RTPCR cDNA Subtraction | 601 |
In Situ Hybridization of Radioactive Riboprobes to RNA in Tissue | 611 |
In Situ Hybridization to RNA in Whole Embryos | 623 |
Wholemount In Situ Hybridization to Xenopus Embryos | 635 |
Immunohistochemistry Using Polyester | 655 |
Gene Interference Using Antisense Oligodeoxynucleotides | 673 |
Immunoprecipitation In Vitro Kinase Assays | 699 |
735 | |
Häufige Begriffe und Wortgruppen
acid aliquots animal antibody astrocyte Biol Biology blastoderm buffer cDNA cell lines chick embryo cloning containing culture developmental diluted dissecting dorsal ectoderm EDTA eggs embryos enzyme ethanol explant filter fluorescent forceps gastrulation genetic glass graft hybridization Incubate induced injection labeled lacZ layer limb bud manipulations marker medium mesoderm method mg/mL microinjection microscope Molecular mouse embryo mRNA mutations NaCl needle neural plate neural tube Note notochord oligo overnight paraformaldehyde pellet Petri dish pipet plasmid prepared primers probe protein Protocols reaction region remove reporter gene resuspend retinoids retroviral Rinse room temperature saline screen sections sequences serum Sigma slides solution somites sperm stage staining sterile store at 20°C Subheading supernatant tion tissue tissue-culture transcription transfer transgenic transgenic mice transplantation trypsin vector vitelline membrane vitro Wash X-gal Xenopus yolk zebrafish µg/mL