Ion Channels: Methods and ProtocolsJames D. Stockand, Mark S. Shapiro Springer Science & Business Media, 04.02.2008 - 216 Seiten Internationally recognized biomedical scientists describe recent technological breakthroughs and demonstrate their use in successful experimental designs. The diverse applications range from the study of allosteric regulation of ion channel activity using a classic mutagenesis approach, to the study of channel subunit stoichiometry using a novel biophysical approach based on fluorescence resonance energy transfer. Highlights include methods for heterologous expression of ion channels in cells, for determining channel structure-function, for studying channel regulation and physiological function, and for genetic screening and investigating channelopathies. |
Inhalt
| 3 | |
Overexpression of Proteins in Neurons Using | 15 |
Exogenous Expression of Proteins in Neurons Using | 27 |
Tertiary and Quaternary Structure Formation of VoltageGated | 41 |
Biophysical Approach to Determine the Subunit Stoichiometry | 53 |
Energy Transfer Analysis | 65 |
Probing the Effects of Phosphoinositides on Ion Channels | 81 |
Bakhrom K Berdiev and Dale J Benos 9 A Simple In Vivo Method for Assessing Changes of Membrane | 89 |
Colbert 11 Juxtacellular Labeling and Chemical Phenotyping | 117 |
Toney and Lynette C Daws 12 Carbon Fiber Amperometry in the Study of Ion | 127 |
DukSu Koh | 139 |
Genetic Screening for Functionality of Bacterial Potassium Channel | 157 |
14KCNQ1 K+ ChannelMediated Cardiac Channelopathies | 167 |
TissueSpecific Transgenic and Knockout Mice | 185 |
| 207 | |
Mouhamed S Awayda Weijian Shao Ivana Vukojicic | 101 |
Andere Ausgaben - Alle anzeigen
Ion Channels: Methods and Protocols James D. Stockand,Mark S. Shapiro Keine Leseprobe verfügbar - 2006 |
Ion Channels: Methods and Protocols James D. Stockand,Mark S. Shapiro Keine Leseprobe verfügbar - 2010 |
Häufige Begriffe und Wortgruppen
aliquots amiloride amperometric antibody approx assay bath solution bilayer Biol bombardment brain buffer Ca2+ carbon fiber cDNA chamber CHO cells clones containing Cre recombinase cRNA crosslinking culture cysteines diluted edited electrode electroporation emission ENaC enzyme epithelial Na+ channel epithelial sodium channel ethanol exocytosis filter fluorescent protein FRET gene green fluorescent protein HEPES imaging Incubate injection intracellular Invitrogen ion channels isoform juxtacellular labeling KCNQ1 lipid loading loxP medium membrane Methods and Protocols mice microcarriers microscope Molecular mouse MthK mutant NaCl neurons Neurosci Note oocytes patch clamp pellet perfusion Petri dish phosphoinositides Physiol pipet plasmid plates polylysine potassium channel reagents recombination recording resuspend room temperature sample sequence Sigma slices sodium step Stockand stoichiometry Subheading subunit stoichiometry targeting tion tissue transfected transfer transgenic tube vector vesicles virion voltage vortex Western blot whole-cell