Neurons: Methods and Applications for the Cell BiologistElsevier, 02.10.2003 - 500 Seiten Neurons: Methods and Applications for the Cell Biologist lays out numerous simple techniques for growing and carrying out experiments with many varieties of neurons. Subjects include peripheral and central neurons from vertebrate and invertebrate sources, as well as neuron-like cell lines. It also explains recent advances in our ability to introduce exogenous proteins and genes to neurons in culture. Procedures for successful protein infiltration, biolistic transfection, electroporation, and viral transgenic methods in neurons are also presented.
|
Inhalt
| 1 | |
| 17 | |
A System Well Suited to Synaptic Study | 37 |
Chapter 4 The Culture of Chick Forebrain Neurons | 51 |
Chapter 5 Growing and Working with Spinal Motor Neurons | 67 |
Extrinsic Control of Morphology by Cell Type and Glutamate | 89 |
Chapter 7 Culturing Hippocampal and Cortical Neurons | 111 |
Chapter 8 Working with Xenopus Spinal Neurons in Live Cell Culture | 129 |
Chapter 12 PC12 Cells as a Model for Studies of Regulated Secretion in Neuronal and Endocrine Cells | 267 |
An Easily Transfected Cultured Cell Model of Central Nervous System Neurons | 287 |
Chapter 14 LiveCell Imaging of Slow Axonal Transport in Cultured Neurons | 305 |
Assisted Delivery of Proteins and Peptides into Living Neurons | 325 |
Chapter 16 Tranfection of Primary Central and Peripheral Nervous System Neurons by Electroporation | 339 |
Chapter 17 Biolistic Transfection | 353 |
Chapter 18 Expression of Transgenes in Primary Neurons from Chick Peripheral and Central Nervous Systems by Retroviral Infection of Early Embr... | 369 |
Chapter 19 Production and Use of ReplicationDeficient Adenovirus for Transgene Expression in Neurons | 387 |
Chapter 9 Culturing Neurons from the Snail Helisoma | 157 |
Chapter 10 The Tibial1 Pioneer Pathway An in Vivo Model for Neuronal Outgrowth and Guidance | 171 |
Chapter 11 Techniques to Dissect Cellular and Subcellular Function in the Drosophila Nervous System | 195 |
| 417 | |
Volume in Series | 435 |
Andere Ausgaben - Alle anzeigen
Neurons: Methods and Applications for the Cell Biologist, Band 71 Peter J. Hollenbeck,James R. Bamburg Eingeschränkte Leseprobe - 2003 |
Häufige Begriffe und Wortgruppen
adenovirus antibodies assay astrocytes axonal transport B35 cells basal lamina brain Broadie buffer Ca2+ calcium Cell Biol Cell Biology cell body cell lines chick forebrain neurons ciliary clones containing cortical culture dishes cultured neurons cytosol dendrites density dissection dissociated dorsal Drosophila melanogaster eggs electroporation embryo exocytosis explant expression extracellular fluorescent forceps function g/ml ganglia ganglion gene genetic glass coverslips glia glutamate glutamate transporters growth cone HBSS hippocampal hippocampal neurons imaging incubator injection insert laminin membrane methods micropipette microscope microtubules Molecular molecules motility motor neurons mutants nervous system neurite neurofilament neuronal cell neuronal cultures Neurosci nonneuronal cells O’Connor outgrowth particles PC12 cells peptide permeable pipette plasmid plate poly-d-lysine protein protocol Purkinje neurons Rabbit pAB reagents receptor recombinant removed rinse serum solution spinal cord sterile studies substrate synaptic vesicle tissue culture transfection transgene vectors ventral viral virus vitro vivo volume Xenopus
Beliebte Passagen
Seite 16 - Identification of a novel force-generating protein, kinesin, involved in microtubule-based motility.