Advanced Bacterial Genetics: Use of Transposons and Phage for Genomic EngineeringElsevier, 27.02.2007 - 320 Seiten The critically acclaimed laboratory standard for more than fifty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with over 400 volumes (all of them still in print), the series contains much material still relevant today—truly an essential publication for researchers in all fields of life sciences. This new volume presents methods related to the use of bacterial genetics for genomic engineering. The book includes sections on strain collections and genetic nomenclature; transposons; and phage. |
Andere Ausgaben - Alle anzeigen
Advanced Bacterial Genetics: Use of Transposons and Phage for ..., Band 421 Kelly Thomas Hughes,Stanley R. Maloy Keine Leseprobe verfügbar - 2007 |
Häufige Begriffe und Wortgruppen
acid agar allele analysis antibiotic antibiotic resistance assay auxotrophic bacteria bacterial Bacteriol bacteriophage binding Biol cassette cells centrifuge challenge phage chromosomal DNA cloning colonies containing deletions diluted DNA‐binding duplication electroporation encodes Enzyme Escherichia coli essential genes expression flanking fragment g/ml gene fusions gene of interest genetic genome homologous recombination homology hybridization Incubate overnight insertion mutants IPTG isolate lac operon lacZ lysate lysogens Maloy Manoil marker medium membrane method microarray Microbiol Molecular Mud insertion MudJ or MudK mutagenesis mutagenized mutant libraries Natl oligo operon overnight at 37 overnight culture P22 phage PCR product pellet phenotype plasmid plates pool primers promoter prophage protein protocol reaction region replication Salmonella enterica screen selection sequence specific sterile T‐POP target tetA tetR tetRA element tetracycline TEV protease Tn10 insertion Tn10dTc‐araCþ transcription transducing transposase transposition transposon transposon insertion tube typhimurium vitro vivo VOLUME wild‐type
Beliebte Passagen
Seite xxiv - VOLUME 356. Laser Capture Microscopy and Microdissection Edited by P. MICHAEL CONN VOLUME 357. Cytochrome P450, Part C Edited by ERIC F. JOHNSON AND MICHAEL R. WATERMAN VOLUME 358. Bacterial Pathogenesis (Part C: Identification, Regulation, and Function of Virulence Factors) Edited by VIRGINIA L. CLARK AND PATRIK M. BAVOIL VOLUME 359. Nitric Oxide (Part D) Edited by ENRIQUE CADENAS AND LESTER PACKER VOLUME 360. Biophotonics (Part A) Edited by GERARD MARRIOTT AND IAN PARKER VOLUME 361. Biophotonics...
Seite xxv - DOYLE AND ITZHAK OFEK VOLUME 254. Oncogene Techniques Edited by PETER K. VOGT AND INDER M. VERMA VOLUME 255. Small GTPases and Their Regulators (Part A: Ras Family) Edited by WE BALCH, CHANNING J. DER, AND ALAN HALL VOLUME 256. Small GTPases and Their Regulators (Part B: Rho Family) Edited by...
Seite xxxii - Edited by Jo M. HOLT, MICHAEL L. JOHNSON, AND GARY K. ACKERS VOLUME 380. Energetics of Biological Macromolecules (Part E) Edited by Jo M.
Seite xxi - VOLUME 178. Antibodies, Antigens, and Molecular Mimicry Edited by JOHN J. LANGONE VOLUME 179. Complex Carbohydrates (Part F) Edited by VICTOR GINSBURG VOLUME 180. RNA Processing (Part A: General Methods) Edited by JAMES E. DAHLBERG AND JOHN N. ABELSON VOLUME 181. RNA...
Seite xxxi - Function of Virulence Factors) Edited by VIRGINIA L. CLARK AND PATRIK M. BAVOIL VOLUME 359. Nitric Oxide (Part D) Edited by ENRIQUE CADENAS AND LESTER PACKER VOLUME 360. Biophotonics (Part A) Edited by GERARD MARRIOTT AND IAN PARKER VOLUME 361. Biophotonics (Part B) Edited by GERARD MARRIOTT AND IAN PARKER VOLUME 362. Recognition of Carbohydrates in Biological Systems (Part A) Edited by YUAN C. LEE AND REIKO T. LEE VOLUME 363. Recognition of Carbohydrates in Biological Systems (Part B) Edited by...
Seite xxxii - VOLUME 378. Quinones and Quinone Enzymes (Part A) Edited by HELMUT SIES AND LESTER PACKER VOLUME 379. Energetics of Biological Macromolecules (Part D) Edited by Jo M.