Reporter Genes: A Practical GuideDonald S. Anson Springer Science & Business Media, 13.09.2007 - 158 Seiten Reporter genes have played, and continue to play, a vital role in many areas of biological research by providing a ready means for qualitative and quantitative assessment of the activity of genes and location of gene products in different environments. In Reporter Genes: A Practical Guide, renowned researchers describe practical protocols for experimentation with the most useful reporter genes for mammalian systems that are available, concentrating on those marker genes that are currently most commonly used. Among the topics covered in this volume are methodologies for staining and visualization of ß-galactosidase in embryos and tissues, immunohistochemical detection of ß-galactosidase, detection of reporter gene expression in murine airways, and three dimensional analysis of molecular signals with episcopic imaging techniques. Also covered in this groundbreaking text are fluorescent proteins, detection of GFP during nervous system development, fluorescent protein reporter systems for single cell measurements, and non-invasive imaging of molecular events. Comprehensive and illuminating, Reporter Genes: A Practical Guide will be an oft-used reference for geneticists, molecular engineers, and molecular biologists. |
Inhalt
Immunohistochemical Detection of βGalactosidase | 13 |
Detection of Reporter Gene Expression in Murine Airways | 25 |
ThreeDimensional Analysis of Molecular Signals | 35 |
Wolfgang J Weninger and Timothy J Mohun 5 Fluorescent Proteins for Cell Biology 135 | 47 |
Detection of GFP During Nervous System Development | 81 |
Autofluorescent Proteins for Flow Cytometry | 99 |
Fluorescent Protein Reporter Systems for SingleCell Measurements | 111 |
Subcellular Imaging of Cancer Cells in Live Mice | 121 |
Noninvasive Imaging of Molecular Events with Bioluminescent | 131 |
The Power of Vapor Fixation | 145 |
155 | |