Plant Functional Genomics: Methods and ProtocolsErich Grotewold Springer Science & Business Media, 03.02.2008 - 452 Seiten Functional genomics is a young discipline whose origin can be traced back to the late 1980s and early 1990s, when molecular tools became available to determine the cellular functions of genes. Today, functional genomics is p- ceived as the analysis, often large-scale, that bridges the structure and organi- tion of genomes and the assessment of gene function. The completion in 2000 of the genome sequence of Arabidopsis thaliana has created a number of new and exciting challenges in plant functional genomics. The immediate task for the plant biology community is to establish the functions of the approximately 25,000 genes present in this model plant. One major issue that will remain even after this formidable task is c- pleted is establishing to what degree our understanding of the genome of one model organism, such as the dicot Arabidopsis, provides insight into the or- nization and function of genes in other plants. The genome sequence of rice, completed in 2002 as a result of the synergistic interaction of the private and public sectors, promises to significantly enrich our knowledge of the general organization of plant genomes. However, the tools available to investigate gene function in rice are lagging behind those offered by other model plant systems. Approaches available to investigate gene function become even more limited for plants other than the model systems of Arabidopsis, rice, and maize. |
Inhalt
3 | |
Using Methylation Filtration Technology | 21 |
Strategies and Pitfalls in Expression Analysis from a Few Cells | 59 |
Using GC Counting to Determine the Species of Origin | 79 |
Computer Software to Find Genes in Plant Genomic | 87 |
Genomic Colinearity as a Tool for Plant Gene Isolation | 109 |
Sherry R Whitt and Edward S Buckler IV 9 Quantitative Trait Locus Analysis as a Gene Discovery Tool | 123 |
Transposon Tagging Using Activator Ac in Maize | 157 |
Exploring the Potential of Plant RNase | 295 |
Maintaining Collections of Mutants for Plant Functional Genomics | 311 |
Vector Construction for Gene Overexpression | 329 |
Johan Memelink | 345 |
Expression Profiling Using cDNA Microarrays | 365 |
of Plant Transcriptomes and Gene Discovery | 381 |
Ulrike Mathesius Nijat Imin Siria H A Natera | 395 |
Metabolite Profiling as a Functional Genomics Tool | 415 |
Marcela RojasPierce and Patricia S Springer 15 HighThroughput TAILPCR as a Tool to Identify | 221 |
Custom KnockOuts with Hairpin RNAMediated Gene Silencing | 273 |
Rajendra Marathe Michael Schiff and Yule Liu | 287 |
Susanne Kjemtrup Douglas C Boyes Cory Christensen | 427 |
443 | |
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Acad acid agarose gel Agrobacterium alignment allele amplify analysis approx Arabidopsis thaliana Biol buffer cDNA cDNA fragments centrifuge chromosome cloning coli column containing cycles database detected digestion dilution electrophoresis electroporation elements ethanol exons flanking gene expression gene prediction GeneCalling GeneMark.hmm genetic genomic DNA genomic sequence Genscan growth stage high-throughput HT-TAIL-PCR hybridization identify Incubate insertion Intr introns Invitrogen kanamycin laboratory ligation lines maize mapping markers Methods and Protocols microarray mixture Molecular mRNA mutagen mutagenesis mutation Mzef Natl Note nucleotide overexpression PCR product pellet phenotype Plant Cell plasmid plate polymerase population primer probes protein Proteomics Qiagen reaction regions RescueMu restriction enzyme Resuspend rice RNase room temperature samples screen seeds selection solution specific step Subheading T-DNA TAIL-PCR target mRNA TBE buffer thermal cycler tion tissue trait transcription transformation transgenic transposable transposon Tris-HCl tube vector wash