Neurons: Methods and Applications for the Cell Biologist

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Peter J. Hollenbeck, James R. Bamburg
Gulf Professional Publishing, 22.07.2003 - 441 Seiten
Written by cell biologists and neuroscientists, this volume in the Methods in Cell Biology series is a detailed guide for cell biologists who would like to approach their experimental questions using nature's most interesting cells - neurons. Many cell biologists study the properties of cells grown outside of the animal in the controlled environment of a culture dish, but they would benefit from directing their attention to nerve cells as they are well-suited to almost every area of cell biological inquiry.
This book lays out numerous simple techniques for growing and carrying out experiments with many varieties of neurons. Subjects include peripheral and central neurons from vertebrate and invertebrate sources, as well as neuron-like cell lines. It also explains recent advances in our ability to introduce exogenous proteins and genes into neurons in culture. Procedures for successful protein infiltration, biolistic transfection, electroporation, and viral transgenic methods in neurons are also presented.
With the current and reliable information contained in this volume, growing various kinds of neurons in culture and using them for diverse experimental applications will be easy for cell biologists to master!

* Contains culture methodology for more than a dozen types of CNS and PNS neurons
* Includes most recent and reliable techniques from expert practitioners for specific experimental applications
* Addresses the latest strategies for transfecting neurons
 

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Inhalt

Working with Xcnopus Spinal Neurons in Live Cell Culture
8
PC 12 Cells as a Model for Studies of Regulated Secretion in Ncuronal
12
Growing and Working with Peripheral Neurons
17
A System
37
The Culture of Chick Forebrain Neurons
51
Growing and Working with Spinal Motor Neurons
67
Extrinsic Control of Morphology
89
Culturing Hippocampal and Cortical Neurons
111
Techniques to Dissect Cellular and Subcellular Function in the Drosophila
195
An Easily Transfected Cultured Cell Model
287
LiveCell Imaging of Slow Axonal Transport in Cultured Neurons
305
Assisted Delivery of Proteins and Peptides into
325
Tranfection of Primary Central and Peripheral Nervous System Neurons
339
Biolistic Transfection
353
Expression of Transgenes in Primary Neurons from Chick Peripheral
369
Production and Use of ReplicationDeficient Adenovirus for Transgene
387

Culturing Neurons from the Snail Hclisoma
157
An in Vivo Model for Neuronal Outgrowth
171

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