Neurons: Methods and Applications for the Cell Biologist, Band 71Peter J. Hollenbeck, James R. Bamburg Gulf Professional Publishing, 08.07.2003 - 441 Seiten Neurons: Methods and Applications for the Cell Biologist lays out numerous simple techniques for growing and carrying out experiments with many varieties of neurons. Subjects include peripheral and central neurons from vertebrate and invertebrate sources, as well as neuron-like cell lines. It also explains recent advances in our ability to introduce exogenous proteins and genes to neurons in culture. Procedures for successful protein infiltration, biolistic transfection, electroporation, and viral transgenic methods in neurons are also presented.
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Inhalt
The Culture of Chick Forebrain Neurons | 4 |
A System | 37 |
Steven R Heidemann Matthew Reynolds Kha Ngo and Phillip Lamoureux | 51 |
Growing and Working with Spinal Motor Neurons | 67 |
22845 | 80 |
18 | 86 |
Extrinsic Control of Morphology | 89 |
31 | 106 |
Techniques to Dissect Cellular and Subcellular Function in the Drosophila | 195 |
38 | 211 |
PC12 Cells as a Model for Studies of Regulated Secretion in Neuronal | 267 |
An Easily Transfected Cultured Cell Model | 287 |
Materials | 297 |
LiveCell Imaging of Slow Axonal Transport in Cultured Neurons | 305 |
Assisted Delivery of Proteins and Peptides into | 325 |
Tranfection of Primary Central and Peripheral Nervous System Neurons | 339 |
Culturing Hippocampal and Cortical Neurons | 111 |
Working with Xenopus Spinal Neurons in Live Cell Culture | 129 |
34 | 138 |
Culturing Neurons from the Snail Helisoma | 157 |
An in Vivo Model for Neuronal Outgrowth | 171 |
Biolistic Transfection | 353 |
Expression of Transgenes in Primary Neurons from Chick Peripheral | 369 |
Production and Use of ReplicationDeficient Adenovirus for Transgene | 387 |
Methods | 411 |
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adhesion antibodies assay astrocytes B35 cells basal lamina Biology brain Broadie buffer Ca2+ Cell Biol cell body cell lines chick forebrain neurons ciliary containing cortical culture dishes cultured neurons cytosol dendrites density differentiation dissection dissociated dorsal Drosophila melanogaster eggs electroporation embryo exocytosis explant expression extracellular filopodia fluorescent forceps function ganglia ganglion gene genetic glass coverslips glia glutamate transporters grasshopper growth cone HBSS hippocampal hippocampal neurons imaging incubator injection laminin limb bud membrane methods mg/ml micropipette microscope microtubules Molecular molecules morphology motility motor neurons mutants nerve nervous system neural tube neurite neuronal cell neuronal cultures Neurosci nonneuronal cells outgrowth particles Pasteur pipette PC12 cells peptide permeable pipette plating poly-D-lysine protein protocol Purkinje cells Purkinje neurons Rabbit pAB receptor removed rinse serum solution spinal cord stage sterile studies substrate sympathetic tissue culture transfection transgene vectors ventral virus vitro vivo Xenopus µg/ml