Microinjection: XD-US. ...Juan Carlos Lacal, Rosario Perona, James Feramisco Springer Science & Business Media, 1999 - 248 Seiten 1. Needle Microinjection: A Brief History.- 2. Microinjection of Macromolecules into Mammalian Cells in Culture.- 3. The Use of Plasmid Microinjection to Study Specific Cell Cycle Phase Transitions.- 4. Combining Microinjection with Microanalytical Methods to Investigate Regulation of Metabolic Pathways.- 5. Import of Stably Folded Proteins into Peroxisomes.- 6. Study of the Function of Microtubule Proteins.- 7. Cytoskeleton Regulation by Rho Proteins.- 8. Cytoskeletal Regulation by Rho-p21 Associated Kinases: Analysis of ROK and PAK Function by Plasmid Microinjection.- 9. Use of Microinjection to Study Apoptosis and its Prevention.- 10. Role of the InsP3 Receptor in Intracellular Ca2+ Release and Ca2+ Entry.- 11. Microinjection of Xenopus laevis Oocytes: A Model System.- 12. Xenopus laevis Oocytes as a Model for Studying the Activation of Intracellular Kinases.- 13. Signal Transduction in Xenopus laevis Oocytes by Ras Oncoproteins and Lipid Metabolites.- 14. The Multiple Roles of Mos During Meiosis.- 15. Use of the Xenopus Oocyte System to Study RNA Transport.- 16. Functional Expression of G Protein-Coupled Receptors in Xenopus laevis Oocytes.- 17. Expression of Glucose Transporters in Xenopus laevis Oocytes: Applications for the Study of Membrane Transporter Function and Regulation.- 18. DNA Injection into Xenopus laevis Embryos as a Tool to Study Spatial Gene Activity.- 19. Transformation of Nematodes by Microinjection.- Guide to Solutions.- Guide to Protocols.- Troubleshooting Guide. |
Inhalt
II | 9 |
16 | |
V | 17 |
VII | 18 |
IX | 19 |
X | 20 |
XI | 21 |
XV | 22 |
CXIX | 113 |
CXX | 115 |
CXXII | 116 |
CXXIII | 117 |
CXXV | 118 |
CXXVI | 119 |
CXXVIII | 120 |
CXXIX | 121 |
XVI | 23 |
XVII | 24 |
XX | 25 |
XXI | 26 |
XXIV | 27 |
XXV | 28 |
XXVI | 29 |
XXIX | 30 |
31 | |
XXXI | 33 |
XXXII | 34 |
XXXIII | 35 |
XXXV | 37 |
XXXVIII | 39 |
XL | 41 |
44 | |
XLIV | 47 |
XLVI | 48 |
XLVIII | 51 |
L | 53 |
LI | 57 |
LII | 58 |
LIII | 59 |
60 | |
LVI | 61 |
LVII | 62 |
LX | 63 |
LXIII | 64 |
LXV | 65 |
LXVI | 66 |
LXVII | 67 |
LXVIII | 69 |
LXIX | 70 |
LXX | 71 |
72 | |
LXXIV | 74 |
LXXV | 75 |
LXXVI | 76 |
LXXVII | 77 |
LXXX | 78 |
LXXXI | 79 |
81 | |
LXXXIV | 82 |
LXXXV | 83 |
LXXXVIII | 84 |
LXXXIX | 85 |
XC | 86 |
XCI | 88 |
XCII | 91 |
XCIII | 92 |
94 | |
XCVII | 95 |
XCVIII | 96 |
XCIX | 97 |
C | 98 |
CI | 99 |
CII | 100 |
CIV | 101 |
CVI | 102 |
CVII | 104 |
CVIII | 105 |
106 | |
CXII | 107 |
CXIII | 108 |
CXV | 110 |
CXVII | 111 |
CXVIII | 112 |
123 | |
CXXXII | 125 |
CXXXIII | 126 |
CXXXV | 127 |
CXXXVIII | 128 |
CXLI | 130 |
CXLII | 131 |
CXLIV | 132 |
134 | |
CXLVIII | 135 |
CXLIX | 138 |
CL | 139 |
CLII | 140 |
CLIII | 142 |
CLV | 143 |
CLVI | 144 |
146 | |
CLXI | 149 |
CLXIII | 151 |
CLXVII | 152 |
CLXVIII | 153 |
CLXX | 154 |
CLXXII | 155 |
CLXXIII | 156 |
CLXXIV | 157 |
CLXXV | 158 |
CLXXVII | 159 |
161 | |
CLXXXI | 163 |
CLXXXII | 165 |
CLXXXIII | 167 |
CLXXXIV | 168 |
CLXXXV | 170 |
CLXXXVII | 174 |
187 | |
CXC | 190 |
CXCI | 191 |
CXCII | 193 |
CXCV | 194 |
CXCVIII | 195 |
CCI | 196 |
CCII | 197 |
CCIII | 198 |
CCIV | 199 |
211 | |
CCVII | 212 |
CCVIII | 213 |
CCX | 214 |
CCXI | 216 |
CCXII | 217 |
CCXV | 218 |
CCXVI | 219 |
CCXVII | 220 |
223 | |
CCXX | 225 |
CCXXI | 226 |
CCXXII | 227 |
CCXXIV | 228 |
CCXXV | 229 |
CCXXVI | 231 |
233 | |
CCXXIX | 234 |
CCXXX | 235 |
CCXXXI | 238 |
CCXXXIII | 240 |
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2-deoxyglucose-6-P antibody antisense aphidicholin apoptosis arrest assay binding Biochem Biol Chem blocking solution BrdU buffer Solution c-myc Ca2+ entry capillary Carnero Cdc42Hs cDNA Cell Biol cell cycle cellular cloning concentration coverslips cyclin cytes cytoplasmic dehydrogenase EMBO embryos endogenous enzyme expression plasmid factor Feramisco fibroblasts fluorescence function gene glucose transport glucose-6-P glycogen synthase Graessmann GTPase GVBD heparin incubate induced inhibit inhibitor injected cells intracellular jected Lacal LTAg mammalian cells MAP kinase MAPK measured meiosis meiotic membrane mg/ml microin microinjection microscope microtubule Molecular Mos protein mouse mRNA mutant myocytes needle nematode ng/ml nuclear oncogene oocyte maturation oocytes pathway PDGF peptide peroxisomal phosphorylation pipet plasmid progesterone protein kinase Protocol Ras proteins reaction Reagent receptor regulation RhoA S-phase Sagata sample serum Sigma signal specific staining substrate subunit synthesis tein tion vitro Wash cells Xenopus laevis Xenopus oocytes µg/ml
Beliebte Passagen
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