Live Cell Imaging: A Laboratory Manual, Band 1

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Robert D. Goldman, David L. Spector
CSHL Press, 2005 - 631 Seiten
Recent advances in imaging technology reveal, in real time and great detail, critical changes in living cells and organisms. This manual is a compendium of emerging techniques, organized into two parts: specific methods such as fluorescent labeling, and delivery and detection of labeled molecules in cells; and experimental approaches ranging from the detection of single molecules to the study of dynamic processes in organelles, organs, and whole animals. Although presented primarily as a laboratory manual, the book includes introductory and background material and could be used as a textbook in advanced courses. It also includes a DVD containing movies of living cells in action, created by investigators using the imaging techniques discussed in the book. The editors, David Spector and Robert Goldman, whose previous book was Cells: A Laboratory Manual,are highly respected investigators who have taught microscopy courses at Cold Spring Harbor Laboratory, the Marine Biology Laboratory at Woods Hole, and Northwestern University.
 

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Inhalt

Singlecell Imaging in Animal Tumors In Vivo
409
Chapter 22
410
Longterm Highresolution Imaging in the Neocortex In Vivo
423
Intravital Microscopy of Normal and Diseased Tissues in the Mouse
435
Chapter 24
436
Development of Mammalian Cell Lines with lac Operatortagged
467
Imaging Gene Expression in Living Cells
483
Studying Mitosis in Cultured Mammalian Cells
491

CCD Cameras for Fluorescence Imaging of Living Cells
87
Photobleaching Techniques to Study Mobility and Molecular Dynamics
101
FRET and Fluorescence Lifetime Imaging Microscopy
127
Monitoring Protein Dynamics Using FRETbased Biosensors
145
Chapter 9
155
Application of Lightdirected Activation of Caged Biomolecules and CALI
159
Chapter 13
206
Analyzing Live Cell Data and Tracking Dynamic Movements
303
Chapter 18
326
IMAGING OF LIVE CELLS AND ORGANISMS
327
Chapter 20
357
Timelapse Cinematography in Living Drosophila Tissues
385
Chapter 21
386
Imaging Intermediate Filament Proteins in Living Cells
523
Methods for Expressing and Analyzing GFPTubulin
537
Chapter 31
538
Imaging of Organelle Membrane Systems and Membrane Traffic
555
Imaging Live Cells Under Mechanical Stress
569
Chapter 33
570
Imaging Single Molecules Using Total Internal Reflection Fluorescence
585
Chapter 26
600
Visualization and Quantification of Single RNA Molecules in Living Cells
603
Chapter 35
604
An interactive spreadsheet containing all the steps
614
Cautions
617
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