Microinjection

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Juan Carlos Lacal, Rosario Perona, James Feramisco
Birkhäuser, 01.12.2013 - 248 Seiten
This manual describes the methodology and applications of microinjection, a powerful technique for transposing material into living cells through needle injection. Information is presented on the techniques principles, the required equipment, preparation of receiving cells and injected material, and the analysis of results. Detailed protocols for a wide range of applications in different cellular systems are included. A large part of the book is also dedicated to the Xenopus laevis oocyte system.
 

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Inhalt

Microinjection of Macromolecules into Mammalian Cells in Culture
17
References
30
The Use of Plasmid Microinjection to Study Specific Cell Cycle
31
Combining Microinjection with Microanalytical Methods
44
Import of Stably Folded Proteins into Peroxisomes
60
Study of the Function of Microtubule Proteins
72
Cytoskeleton Regulation by Rho Proteins
81
Use of Microinjection to Study Apoptosis and its Prevention
106
Xenopus laevis Oocytes as a Model for Studying the Activation
146
Signal Transduction in Xenopus laevis Oocytes by
161
The Multiple Roles of Mos During Meiosis
174
Use of the Xenopus Oocyte System to Study RNA Transport
187
Functional Expression of G ProteinCoupled Receptors
199
Expression of Glucose Transporters in Xenopus laevis
211
DNA Injection into Xenopus laevis Embryos as a Tool to Study
223
Transformation of Nematodes by Microinjection
233

Role of the InsP3 Receptor in Intracellular Caº Release
123
A Model System
134
Guide to Solutions
241
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