MicroinjectionJuan Carlos Lacal, Rosario Perona, James Feramisco Birkhäuser, 01.12.2013 - 248 Seiten This manual describes the methodology and applications of microinjection, a powerful technique for transposing material into living cells through needle injection. Information is presented on the techniques principles, the required equipment, preparation of receiving cells and injected material, and the analysis of results. Detailed protocols for a wide range of applications in different cellular systems are included. A large part of the book is also dedicated to the Xenopus laevis oocyte system. |
Inhalt
R Perona F Dolfi J Feramisco and J C Lacal | 17 |
Microinjection of Macromolecules into Mammalian Cells in Culture | 23 |
References | 30 |
The Use of Plasmid Microinjection to Study Specific Cell Cycle | 31 |
Combining Microinjection with Microanalytical Methods | 44 |
Import of Stably Folded Proteins into Peroxisomes | 60 |
Study of the Function of Microtubule Proteins | 72 |
Cytoskeleton Regulation by Rho Proteins | 81 |
Xenopus laevis Oocytes as a Model for Studying the Activation | 146 |
Signal Transduction in Xenopus laevis Oocytes by | 161 |
The Multiple Roles of Mos During Meiosis | 174 |
Use of the Xenopus Oocyte System to Study RNA Transport | 187 |
Functional Expression of G ProteinCoupled Receptors | 199 |
Expression of Glucose Transporters in Xenopus laevis | 211 |
DNA Injection into Xenopus laevis Embryos as a Tool to Study | 223 |
Transformation of Nematodes by Microinjection | 233 |
Use of Microinjection to Study Apoptosis and its Prevention | 106 |
Role of the InsP3 Receptor in Intracellular Ca2+ Release | 123 |
A Model System | 134 |
Andere Ausgaben - Alle anzeigen
Microinjection: XD-US. ... Juan Carlos Lacal,Rosario Perona,James Feramisco Eingeschränkte Leseprobe - 1999 |
Häufige Begriffe und Wortgruppen
2-deoxyglucose-6-P Acad antibodies aphidicholin apoptosis arrest assay binding Biochem Biol Chem BrdU buffer Solution c-myc Ca² Ca2+ entry capillary Carnero Cdc42Hs cDNA Cell Biol Cell culture cell cycle cellular cloning concentration coverslips cyclin cytes cytoplasmic EMBO embryos endogenous expression plasmid coding fibroblasts fluorescence function gene glucose transport glucose-6-P glycogen synthase Graessmann GTPase GVBD heparin immunofluorescence incubate induced inhibit inhibitors injected cells Lacal LTAg mammalian cells MAP kinase MAPK meiosis meiotic membrane mg/ml microinjection microscope microtubule Mos protein mRNA mutant myocytes Natl needle nematode nuclear transport nucleus oncogene oocyte maturation oocytes pathway PDGF peptide peroxisomal phosphorylation pipet plasmid progesterone protein kinase Protocol Ras proteins receptor regulation S-phase sample serum Sigma signal specific staining substrate subunit synthesis teins tion Vande Woude vitro Wash cells Xenopus laevis Xenopus laevis oocytes Xenopus oocytes µg/ml